Alaabta

brucellae waa wakiilada etiological ee brucellosis, zoonosis adduunka oo dhan qaybsan.Bakteeriyadani waa dulin-nololeedka ku-meel-gaadhka ah ee facultative sidaas darteed waxay awoodaan inay la qabsadaan dheef-shiid kiimikaadkooda si ay ula qabsadaan deegaannada ka baxsan-iyo gudaha gudaha ee ay la kulmaan inta lagu jiro wareegga faafa.Si kastaba ha ahaatee, dhinacan bayoolojiga Brucella si aan qummanayn ayaa loo fahmay, iyo nafaqooyinka laga heli karo niche-ga unugyada lama yaqaan.Halkan, waxaan ku baarnay dariiqyada dhexe ee dheef-shiid kiimikaadka C ee uu isticmaalo Brucella abortus anagoo tirtirnay fructose-1,6-bisphosphatase (fbp iyo glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), iyo malic enzyme (mae) hiddo-sideyaasha.Gluconeogenic laakiin maaha warbaahinta hodanka ah, kobaca ΔppdK iyo Δmae mutants aad bay u liidatay waxaana hoos u dhacay kobaca labanlaabka Δfbp-ΔglpX mutant.Makrophages-ka, kaliya ΔppdK iyo Δmae mutants ayaa muujiyay isku dhufashada oo yaraatay, iyo daraasado lagu sameeyay ΔppdK mutant ayaa xaqiijiyay in ay gaartay niche soo noqnoqda.Sidoo kale, kaliya ΔppdK iyo Δmae mutants ayaa lagu dhimay jiirarka, kii hore waxaa la nadiifiyay usbuuca 10 iyo kan dambe wuxuu sii soconayaa in ka badan 12 toddobaad.Waxaan sidoo kale baarnay wareegga glyoxylate.Inkasta oo aceA (isocitrate lyase) dhaqdhaqaaqa dhiirigeliyaha lagu wanaajiyey dhexdhexaadinta qani ah, khalkhalka aceA wax saameyn ah kuma yeelan vitro ama ku dhufashada makrophages ama mouse spleens.Natiijooyinka waxay soo jeedinayaan in B. abortus ay ku koraan intracellularly iyada oo la adeegsanayo sahayda xaddidan ee 6-C (iyo 5-C) sonkorta taas oo ay magdhowdo glutamate iyo suurtogalnimada amino acids kale oo galaya wareegga Krebs iyada oo aan door muhiim ah ka lahayn shunt glyoxylate.