Alaabta

Dhaqdhaqaaqa noolaha: BCIP (BCIP p-toluidine cusbo; X-phosphate p-toluidine cusbo) waa substrate-ka koromojiinka ee ogaanshaha midabka alkaline phosphatase.

Shiga sunta (Stxs) waxaa soo saara enterohemorrhagic Escherichia coli (EHEC), taas oo keenta infekshannada bini'aadamka oo leh natiijo dhimasho badan.Khadadka unugyada Vero, oo laga soo qaatay kelyaha daayeer cagaaran ee Afrika, waxay u taagan yihiin heerka dahabka ee go'aaminta saamaynta cytotoxic ee Stxs.In kasta oo isticmaalkooda caalamiga ah, aqoonta ku saabsan qaab-dhismeedka saxda ah ee Stx reseptor glycosphingolipids (GSLs) iyo kulankooda ku jira rafts dufanku waa liitaa.Halkan waxaan ku soo bandhigaynaa falanqayn qaabaysan oo dhamaystiran oo ku saabsan Stx reseptor GSLs iyo qaybintooda xuubka u adkaysta saabuunta (DRMs), kuwaas oo laga diyaariyey unugyo Vero-B4 loona adeegsaday sidii raft dufaneed u dhigma.Waxaan u aqoonsannay globotriaosylceramide (Gb3Cer) iyo globotetraosylceramide (Gb4Cer) inay yihiin soo-dhoweeyayaasha GSL ee noocyada hoosaadka Stx1a, Stx2a, iyo Stx2e iyadoo la adeegsanayo ogaanshaha dusha sare ee TLC oo ay weheliso MS.Soo-dhoweeyaha Stx aan caadiga ahayn, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), kaas oo si gaar ah loo aqoonsaday (marka lagu daro Gb3Cer iyo Gb4Cer) Stx2e, si buuxda ayaa loo asteeyay.Lipoforms of Stx reseptor GSLs ayaa la ogaaday in ay inta badan xadhkaha goostaan ​​unugyada ceramide oo ka kooban sphingosine (d18:1) iyo C24:0/C24:1 ama C16:0 fatty acid.Waxaa intaa dheer, isku-dhac la leh calaamadaha dufanka dufanka leh, SM iyo kolestaroolka, ee DRM-yada ayaa soo jeediyay GSL-ka oo leh microdomains xuubka.Daraasadani waxay saldhig u tahay sahaminta dheeraadka ah ee sahaminta waxtarka leh ee xuubka rafcaanka ee Stx reseptors ee dhaawaca dhexdhexaadinta sunta ee unugyada Vero-B4.