4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Lambarka buugaagta | XD900018 |
| Magaca Alaabta | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Qaanuunka molecular | C12H15NO8 |
| Miisaanka Molecular | 301.25 |
| Faahfaahinta Kaydinta | -15 ilaa -20 °C |
| Xeerka Tarifka La Iswaafajiyay | 29400000 |
Tilmaamaha Alaabta
| Muuqashada | Caddaan ilaa Orange ilaa Cagaar budo ilaa crystal |
| Qiimayn | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) waa substrate macmal ah oo ah 4-nitrophenyl (pNP) glycopyranoside si loo ogaado dhaqdhaqaaqa α-galactosidase.Qadarka pNP ee la sii daayay ayaa si weyn u kordhay markii 4-Nitrophenyl α-D-galactopyranoside loo isticmaalay substrate ahaan.
Isticmaalka4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, tirada la xoreeyaypNPfromthe4-Nitrophenyla-D-galactopyranosideis muhiimad sare leh oo ka soo jeeda theotherpNP-glycopyranosidesatpH4.0halkaasnoactivation-kutubta yl (pNP) glycopyranosidessartificial substrates ayaa muujinaya in la xoreeyay galactoseresidues ka4-Nitrophenyla-D-galactopyranosidemainlyatpH4.0, iethepHargeerka halka laga soo saarayo heerka ugu hooseeya ayaa hoos u dhacay.
Substrate of α-D-galactosidase (α-D-Galactosidase).Waxay ku xidhan tahay sidaha borotiinka lactose ee E. coli.Dhexdhexaad u ah α-D-galactose.
Hab degdeg ah oo lagu qiimeeyo waxqabadka lytic ee wakiilada antimicrobial ee ka dhanka ah khamiirka iyo fungi ayaa la sameeyay.Imtixaanku wuxuu ku salaysan yahay sii deynta enzyme intracellular, maltase (alfa-glucosidase).Dhaqdhaqaaqa maltase ee la sii daayay waxaa lagu cabiray midab ahaan soo saarista p-nitrophenol ee p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Dhaqdhaqaaqa lytic ee xeryahooda antimicrobial ee kala duwan ayaa lagu cabiray unugyada khamiirka ama xadhkaha biqilka ee fungi filamentous.Dhaqdhaqaaqa ka-hortagga khamiirka Lytic waxaa lagu ogaan karaa gudaha 20 min ka-soo-baxa 30 darajo C ee ka dhanka ah Saccharomyces cerevisiae, Candida albicans, iyo Cryptococcus neoformans.Dhaqdhaqaaqa anti-fungal ee Lytic ayaa soo muuqday ka dib 2 saacadood oo lagu beeray 30 darajo C oo ka soo horjeeda biqilka xadhkaha Aspergillus niger iyo cinerea Botrytis.Unugyada oo dhan ee khamiirka ama fangaska midkoodna biyo kuma shubin PNPG ku filan 3 saacadood gudahood 30 darajo C si ay u keenaan isbeddel midab la ogaan karo.Dhaqdhaqaaqa lytic ee enzymes (tusaale, Lyticase), antibiyootiga (tusaale, Amphotericin B), iyo nooc bakteeriyada soo saara antibiyootiga ayaa la ogaaday iyadoo la isticmaalayo baaritaanka.Tijaabada ka-hortagga khamiirka ayaa lagu habeeyey qaab 96-well microtiter ah.Labada imtixaanba waxa ay bixiyeen ogaanshaha degdega ah, xasaasiga ah, iyo soo saari karo ee lytic anti-khamiirka iyo hawlaha ka hortagga fungal.
