Alaabta

Chemifluorescence: Molecular luminol waa molecule kiimiko ah oo fluorescent ah oo loo bedeli karo aminophthalic acid dawladeed oo faraxsan iyadoo ay joogaan molecules hydrogen peroxide, taas oo soo saarta fluorescence xooggan.Hydrogen peroxide waa wax soo saarka falcelinno badan oo biooxidative ah, markaa way fududahay in la isku xidho fal-celinta biooxidative-ka iyo sawir-qaadista iyadoo la soo bandhigayo luminol.Tusaale ahaan, baaritaanka gulukooska oxidase/catalase wuxuu ogaan karaa fiirsashada hydrogen peroxide ama gulukooska muunada, iyo waqtiga jawaabta waa 0.5s oo keliya (hab firfircoon).Isku darka maaddooyinka chemiluminescent iyo falcelinta difaaca jirka, iyo muujinta xoogga leh ee qaybaha difaaca ee la ogaaday falcelinta iftiinka, waxaa loo yaqaan 'chemiluminescent immunoassay technology'.Sanadkii 1976, Shmeder wuxuu soo jeediyay immunoassay chemiluminescence immunoassay, iyadoo la adeegsanayo luminol-H202 iyo asalkeeda ABEI sida nidaamka muujinta falcelinta ogaanshaha.Waqtigan xaadirka ah, luminol iyo soosaarayaasheedu waa calaamadaha ugu badan ee loo isticmaalo codsiyada immunoassay ee chemiluminescence.Marka la eego xaaladaha alkaline-ka ah, ee ay katalyayso microperoxidase, tiro badan oo sawir-qaade ah ayaa la sii deyn karaa.Luminol waxaa loo isticmaali karaa WesternBlot ee unugyada difaaca ee HRP-ku suntan iyo ogaanshaha hybridization nucleic acid ee baarayaasha HRP-calaamadeysan.Waxa kale oo loo adeegsadaa baadhitaanka dembi-baadhista casriga ah ee ogaanshaha dhiigga.Qalab loo isticmaali karo sumadeeyaha kiimikada waa in uu buuxiyaa shuruudaha soo socda: dhalidda tirada ee luminescence waa mid sare;astaamaheeda physicochemical waxay u dhigmaan nidaamka daraasadda;falcelinteeda luminescent waa natiijada falcelinta oksaydhka ee walaxda iftiinka;inta u dhaxaysa fiirsashada loo isticmaalo Guddaha aan sunta u ahayn noolaha.Dhowr nooc oo reagents chemiluminescence ah ayaa sida caadiga ah loo isticmaalaa: acridine ester, raadiyaha chemiluminescence oo si weyn loo isticmaalo, waa xarun dabiici ah oo tricyclic ah, oo ay fududahay in la oxidize, falcelinta oksaydhka uma baahna kicin, waxayna sii deysaa photons at 430nm;Luminol iyo isoluminol iyo soosaarayaashooda ayaa ah kiimikooyinka ugu bislaaday.Horraantii 1964kii, Iyadoo laga soo sheegay kiimikooyinka, luminol, isoluminol iyo soosaarkooda ayaa loo isticmaalay kiimikooyinka.Immunoassays waxay gaareen natiijooyin wanaagsan.Ogaanshaha luminescence: dhererka mowjadda fluorescence ee ugu fiican waa 400nm

Chemiluminescence: Habka qiimaynta xasaasiga ah, chemiluminescence ayaa si weyn loo isticmaalaa si loo ogaado xagjirka xorta ah iyo dheef-shiid kiimikaadka falcelinta ee laga soo saaro enzymes, unugyada iyo noolaha noolaha.Iftiinka ay soo saaraan alaabooyinkooda oksaydheynta iyo dheef-shiid kiimikaad ayaa loo isticmaali karaa sawir-qaadeyaal kala duwan.ogaanshaha.Chemiluminescence waxaa si weyn loogu isticmaalaa baaritaanka iyo cilmi baarista antioxidants, sida polyphenols, polysaccharides, flavonoids, iyo anthraquinones, sababtoo ah dareenkeeda, degdegga, hawlgalka fudud iyo qiimaha jaban.Nidaamyada chemiluminescence ee inta badan loo isticmaalo go'aaminta anions superoxide waxaa ka mid ah xanthine oxidase-luminol, pyrogallol-luminol iyo dimethyl sulfoxide-luminol.Kan hore waa hannaan enzymatic ah, kan dambena waa nidaam aan enzymatic ahayn.Nidaamyada chemiluminescence ee go'aaminta radicals hydroxyl inta badan waxaa ka mid ah copper sulfate- khamiir (ama unugyada dhuuxa lafta) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, copper sulfate-o-phenanthroline-ascorbic acid-hydrogen peroxide 5 nidaamyada chemiluminescence, ferrous sulfate-luminol-hydrogen peroxide iyo ferrous sulfate-luminol, dhamaantood waxay ku lug leeyihiin falcelinta Fenton ee caadiga ah si ay u abuuraan radicals hydroxyl, ka dibna ay weeraraan wakiilka luminescent si ay u dhaliyaan kiimiko, kaas oo loo isticmaali karo in lagu cabbiro ka saarista waxyaabaha Dhaqdhaqaaqa xaglaha ogsijiinta ee fal-celisa.Habka tijaabada ah: isticmaal nidaamka chemiluminescence pyrogallol-luminol: luminol waxaa lagu sameeyaa 0.05mol/L xal fiirsashada 0.05mol/L NaOH, lagu kaydiyaa meel mugdi ah, iyo biyo la dubo oo laba jibbaaran ka hor inta aan la isticmaalin Dilute ilaa 1mmol/L, isticmaal 1mmol/ L HCl si aad u samayso 0.01mol/L xal pyrogallol ah, ku kaydi qaboojiyaha 4°C, kuna qas biyo laba jibaaran oo la miiray ilaa 16 jeer 6.25×10-4mol/L inta aan la isticmaalin.0.05mol/L pH10.2Na2CO3-NaHCO3 (oo ka kooban 0.1mmol/LEDTA) ayaa la diyaariyey ka hor inta aan la isticmaalin, waxaana lagu qasi jiray 1mmol/L luminol at 2:1 (jajabka jajabka) si loo sameeyo luminol iyo karbohaydrayt ka hor isku darka tijaabada.Inta lagu jiro cabbirka, ku dur muunadaha 10.0 μL ee xajmiyada kala duwan (0, 0.08, 0.4, 2 iyo 10 mg/mL) unugga iftiinka (oo leh muunadda kaydka sida kantaroolka), ka dibna ku dur 6.25 × 10-4mol/L pyrogallol 0.05 Ugu dambeyntii, 0.94 mL oo ah isku dhafka luminol iyo kaarboonka aashitada ayaa lagu daray si loo bilaabo falcelinta (30 ° C), xoogga iftiinka iftiinka waxaa lagu tiriyaa inta u dhaxaysa 2 s, iyo wadarta guud ee xoojinta iftiinka isku dhafan ee 300 s ayaa la qiyaasay.Xoogga iftiinka asalka ah wuxuu ahaa iftiinka marka pyrogallol aan lagu darin.qiimaha.Intaa waxaa dheer, waxaa jira chemiluminescence, fluorescence spectroscopy iyo NBS-dichlorofluorescein nidaamka chemiluminescence ee go'aaminta kiimikada fluorescence, iwm, faa'iidadooda ugu weyn waa dareen sare.

Waxyaabaha Kiimikada: Budada crystalline jaalaha ah.Si fudud ugu milmi kara lye, ku milmi aashitada, ku dhawaad ​​aan biyo lagu milmi karin, oo aan lagu milmi karin khamriga.Xalalka dhexdhexaadka ah ama waxoogaa aashito ah ayaa muujinaya iftiin buluug ah oo dhalaalaya marka uu soo baxo iftiinka ultraviolet.Barta dhalaalka 329-332℃

Isticmaala: Sida falanqeeye kiimikaad iyo tilmaame.Isticmaal falanqeynta Chemiluminescence reagent ogaanshaha (sida go'aaminta fiilooyinka birta ama dhiigga)

Waxay isticmaashaa: Falanqaynta chemiluminescence, sida: cations biraha, dhiiga iyo glucocorticoids

Waxay isticmaashaa: Tijaabada Luminescence: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; ka dib marka lagu daro H2O2) chemiluminescence reagent iyo tilmaame, sida caadiga ah loo isticmaalo falanqaynta chemiluminescence, sida cations biraha, difaaca dhiigga, iwm.