3- [(3- Cholanidopropyl) dimethylammonio] -1 -propanesulfonate Cas: 75621-03-3 98%
Lambarka buugaagta | XD90075 |
Magaca Alaabta | 3-[(3-Cholanidopropyl) dimethylammonio]-1-propanesulfonate |
CAS | 75621-03-3 |
Qaanuunka molecular | C32H58N2O7S |
Miisaanka Molecular | 614.88 |
Faahfaahinta Kaydinta | Ambient |
La is waafajiyay Xeerka Tacriifada | 29242970 |
Tilmaamaha Alaabta
IR | U dhigma tixraaca |
Khasaare xagga qallajinta | <2% |
TLC | Hal goob |
Nitrojiin | 4.46 - 4.65 |
Muuqashada | budo cad |
Solubility (10% gudaha H2O) | Cad oo aan midab lahayn |
Waxqabadka (0.1M gudaha H2O) | <10us/cm |
Qiimaynta HPLC | >98% |
UV (5% gudaha H2O) A290nm | <0.5 |
UV (5% gudaha H2O) A400nm | <0.02 |
Mawduuca dareeraha ah (waxaa qoray GC) Dimethylformamide | <2.0% |
3-[3- (cholamidopropyl) dimethylamino] propanesulfonic acid isticmaalka cusbada gudaha iyo habka isku-dhafka
Dhaqdhaqaaqa noolaha: CHAPS, oo ka soo jeeda Cholicacid, waa saabuunta zwitterionic oo milmisa borotiinnada xuubabka.CHAPS waxa loo isticmaalaa in lagu dejiyo dhismayaal borotiin-DNA ah oo kala duwan waxana ay sii haysan kartaa hawsha biochemical ee borotiinnada ee xalka.
Isticmaala: CHAPS waa saabuunta zwitterionic-ka oo aan dami-baxayn oo loogu talagalay biochemistry xuubka.Waxa loo isticmaali karaa in lagu milmo borotiinada xuubka iyo carqaladaynta isdhexgalka borotiinka-barootiinka.CHAPS waxay leedahay miisaan unugyo micellar ah oo hooseeya (6,150) iyo fiirsashada micellar ee muhiimka ah (6-10 mM) sidaas darteedna waxaa lagaga saari karaa muunada sifaynta.Waxa kale oo ay ku habboon tahay milmaha borotiinnada ee diiradda saaraya isoelectric iyo 2D electrophoresis.CHAPS waxaa caadi ahaan loo isticmaalaa wadaniga ah (urea-free) isoelectric diiradda oo la ogaaday in ay bixiso xal aad u fiican ee qaar ka mid ah diyaarinta unugyada hoose iyo borotiinada dhirta.Sida caadiga ah fiirsashada inta u dhaxaysa 2-4% (w/v) ayaa loo isticmaalaa jel-ka-fiirsashada isoelectric.
Waxay isticmaashaa: saabuunta zwitterionic oo aan denaturing ahayn oo loogu talagalay biochemistry xuubka, kaas oo loo isticmaali karo in lagu milmo borotiinada xuubabka iyo carqaladaynta isdhexgalka borotiinka-protein;sidoo kale ku haboon milmi borotiinnada ee diirada isoelectric iyo electrophoresis laba-cabbir ah;caadi ahaan loo isticmaalo in aan-denaturing (ma urea) isoelectric diiradda;Soo saarista RNA iyo DNA, ma jiraan hawlo RNase iyo DNA ah
Isticmaala: Surfactant Amphoteric, CHAPS waa saabuunta zwitterionic aan denataynin, borotiinka lysate ee solubilizing borotiinada xuubka iyo isdhexgalka beenta ah ee ka dhexeeya borotiinada iyo borotiinada.