Alaabta

2,3,4,6-Tetrakis-O-trimethylsilyl-D-gluconolactone, oo caadi ahaan loo yaqaan TMS-D-glucose, waa xarun isku dhafan oo ka hela codsiyada qaybaha sayniska ee kala duwan, oo ay ku jiraan isku-darka organic, kiimikada karbohaydraytyada, iyo kiimikada falanqaynta. TMS-D-glucose ayaa si gaar ah qiimo ugu leh isku-dhafka dabiiciga ah maadaama ay u adeegto sidii koox difaac u ah kooxaha shaqada ee hydroxyl (OH) ee karbohaydraytyada.Marka la soo bandhigo kooxaha trimethylsilyl (TMS) ee kooxaha hydroxyl ee gulukooska, xaruntu waxay noqotaa mid deggan oo aan firfircoonayn, taas oo u oggolaanaysa wax ka beddelka xulashada kooxaha hydroxyl ee gaarka ah halka kuwa kale ay ka tagayaan iyada oo aan saameyn ku yeelan inta lagu jiro isbeddelada kiimikada ee xiga.Istaraatiijiyadan ilaalinta-ilaalinta waxaa si weyn loogu isticmaalaa kiimikada karbohaydraytyada si loo gaaro regioselectivity iyo stereochemistry ee isku-dhafka karbohaydraytyada adag, glycoconjugates, iyo alaabta dabiiciga ah karbohaydraytyada.Marka loo beddelo karbohaydraytyada noocyadooda trimethylsilyl, isbeddelkooda iyo degganaanshahooda kulaylku way fiicnaanayaan, taas oo ka dhigaysa inay ku habboon yihiin falanqaynta gaaska chromatography (GC) iyo spectrometry mass (MS).Farsamaynta derivatization Tani waxay wanaajisaa dareenka ogaanshaha, hagaajinaysaa hufnaan kala soocidda, iyo awood aqoonsiga ee karbohaydraytyada kala duwan ee isku dhafka adag, sida muunado noole ama alaabta cuntada.TMS-D-glucose sidoo kale helay codsiyada in synthesis ee reagents gaar ah iyo baaritaan kiimikaad.Falcelinteeda gaarka ah iyo xasilloonida ayaa ka dhigaysa walxo bilaw ah oo qiimo leh oo loogu talagalay diyaarinta xeryahooda kale ee karbohaydrayt-ka-soo-baxa ah.Cilmi-baadhayaashu waxay wax ka beddeli karaan unugyada trimethylsilyl ama waxay bedeli karaan unugyada gulukooska si ay u abuuraan xeryahooda leh sifooyin gaar ah, sida baaritaanka fluorescent, enzyme inhibitors, ama musharaxiinta daroogada.Kala-soo-baxyadan waxaa lagu shaqaaleysiin karaa cilmi-baarisyo kala duwan oo bayooloji iyo bayooloji ah, oo ay ku jiraan sawir-qaadista, horumarinta daroogada, ama fahamka isdhexgalka carbohydrate-protein. Si kastaba ha ahaatee, waa lagama maarmaan in la tixgeliyo in TMS-D-glucose, sida kiimikooyinka kale ee kale, waxay u baahan tahay maarayn habboon iyo badbaado. ka taxadaritaan.Cilmi baadhayaashu waa inay xaqiijiyaan hawo ku filan oo ay isticmaalaan qalabka ilaalinta shakhsi ahaaneed marka ay la shaqaynayaan xaruntan si looga hortago khataraha caafimaad ee iman kara.Intaa waxaa dheer, si la mid ah kiimikaad kasta oo kimikalka ah, nadiifnimada, iyo tayada TMS-D-glucose ayaa muhiim u ah helitaanka natiijooyin la isku halayn karo oo la soo saari karoAwooddeeda si ay si xushmad leh u ilaaliso kooxaha hydroxyl ee karbohaydraytyada, ku habboonaanta falanqaynta karbohaydraytyada, iyo faa'iidada ay u leedahay isku-darka reagents-yada gaarka ah waxay ka dhigtaa aalad muhiim u ah qaybaha sayniska ee kala duwan.Isticmaalka TMS-D-glucose, cilmi-baarayaashu waxay horumarin karaan waxbarashadooda ku saabsan kiimikada karbohaydraytyada, glycoscience, iyo meelaha la xidhiidha, iyaga oo gacan ka geysanaya horumarinta xeryahooda cusub, ogaanshaha, iyo wakiilada daaweynta.